For a— d , the optimal conditions are labeled in red and a shared y axis is used.
First, males orient themselves while playing a courtship song by horizontally extending and vibrating their wings.
Specifically, the mesh basket was lifted from the cryobuffer and blotted on a paper towel to remove as much liquid as possible, followed by a 5—10 s dip in isopropanol until all embryos sank to the bottom.